Journal: bioRxiv

Article Title: cGAS-STING dependent type I IFN protects against Leptospira interrogans renal colonization in mice

doi: 10.1101/2025.06.02.657349

Figure Lengend Snippet: L. interrogans infection of macrophages induces a type I IFN response. (A-D) Quantification of TNF, IL-10, IL-6, and IFNβ by ELISA in culture supernatants of WT BMDMs infected with L. interrogans Fiocruz L1-130 (MOI 100) for 4, 6, and 8h. (E) Immunoblot for p-TBK1, total TBK1, p-IRF3, total IRF3 and β-actin in cell lysates of BMDMs infected with L. interrogans (MOI 100) for 2, 4, and 6h. Data are representative of three independent experiments. (F, G) Relative expression of Ifna and Ifnb transcript levels in BMDMs infected with L. interrogans at 4 and 6h post infection. (H) Relative expression of IFNB transcript levels in PMA-differentiated human THP-1 cells infected with L. interrogans at 4, 6, and 8h post infection. (A-D and F-H) Data are pooled from three independent experiments and expressed as the mean ± SEM.

Article Snippet: Primary antibodies from Cell signaling technologies (CST) were used as follows: TBK1 (CST #3504S), p-TBK1 (CST #5483S), IRF3 (CST #4302S), p-IRF3 (CST #4947S) and β-actin (CST #4970S).

Techniques: Infection, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing